Phytosynthesis, Characterization and Antimicrobial Activity of Iron Oxide Nanoparticle using Henna (Lawsonia Inermis)

Dr. C. M. Noorjahan

Henna Leaves Extract; Iron Oxide Nanoparticle; Synthesis; Characterization; Antimicrobial Activity

Nanotechnology is the most capable technology that can be applied in any field of interest. Technology has created an impressive increase in the production of nanomaterial in many sector of the society. The biosynthesis of nanoparticle is the intersection of nanotechnology and biotechnology that has received increasing attention due to a growing need to develop eco-friendly technologies in material synthesis. Hence an investigation has been carried out to synthesize Iron oxide nanoparticle using Henna leaves (Lawsonia inermis) extract, to study the characterization and antimicrobial activities of it. The results of the green synthesized Iron oxide nanoparticle using Henna leaves extract showed the change of colour from orange to black which may be due to Surface Plasmon Resonance phenomenon. The results of UV analysis of iron oxide nanoparticle revealed the absorbance peak at 425 nm. The results of FTIR analysis revealed the functional groups present in the Iron oxide nanoparticle, displays a number of peaks which reflects the complex nature. The results of XRD analysis showed a high crystallinity of Iron sample level with diffraction angle ranged from 30-80° at 2Ɵ. The shape of synthesized iron oxide nanoparticle was found to be hexagonal and size was 2µm in diameter using SEM. The result of antimicrobial activity (bacteria and fungi) of synthesized Iron oxide nanoparticle indicates a potent antimicrobial activity of nanoparticle.

[1] Benzie, I.F.F and Strain, J.J. 1996. Ferric Reducing Ability of Plasma (FRAP) as a measure of antioxidant power : The FRAP assay. Anal. Biochem., 239 : 70-76.
[2] Bhui, B.H. Sahoo, D.K. Sarkar, G.P.P. De, S.P. and Mirsa, A. 2009. Green synthesis of Silver nanoparticle using latex of Jatophacurcas. Colloids surf. A: Physico chem. Eng. asp, 339: 134-139.
[3] Caroling, G. Mercy Ranjitham, A. Suja, R. and SunitaTiwari. 2013. Invitro evaluation of Antioxidant, Antimicrobial, Anticancer activities and characterization of Brassica oleracea, Var. Bortrytis. Leaves synthesized silver nanoparticle. International Journal of Pharmacy and Pharmaceutical Sciences. 5(4): 239-251.
[4] Dedeh, A. Ciutat, A. Delapierre, M.T. and Bourdineau, J.P. 2014. Impact of Gold nanoparticle on Zebrafish exposed to a spiked sediment, Nanotoxicology. 1(1):1-10.
[5] Habbal, O. A. A. A. Al-Jabri, and A. G. El-Heg, 2013, Antimicrobial properties of Lawsoniainermis (henna): a review, Australian journal of medical Herbalism, volume 19, no. 3, pp. 265-273.
[6] Ipsa Subhankari. and Nayak, P.I. 2013. Synthesis of Copper nanoparticle using SyzgiumAromaticum (Cloves) aqueous extract by using green chemistry. World Journal of Nano Science and Technology, 2(1): 14-17.
[7] Jayalakshmi and Yogamoorthi, A. 2014. Green Synthesis of Copper oxide nanoparticle using aqueous of flowers of Cassia alata and particles characterization. International Journal of Nanomaterials and Biostructures, 4(2): 66-71.
[8] Jayanthi, D. 2015. Biosynthesis, characterization and antibacterial activity of Zinc oxide nanoparticle. (M.Sc., Dissertation) JBAS College, Chennai, 7-13.
[9] Johan, M.R. Wen, K.S. Hawari, N. and Aznan, N.A.K. 2012. Synthesis and characterization of copper iodide nanoparticle via chemical route. International Journal of Electrochemical Science , 12(3):4942-4950.
[10] Mason, C., Vivekandha, S., Misra, M., Kumar, A. and Mohanty. 2012. Switch grass (Panicumvirgatum) extract medicated green synthesis of silver nanoparticle. World Journal of Nano science and Engineering, 2:47-52.
[11] Mekala, J., Rajan, M.R., Ramesh. R. 2016. Green synthesis and characterization of Copper nanoparticle using Tulsi (Ocimum sanctum) leaf extract. Biological Science, 5(2) :25-31.
[12] Rodriguez, J.A., Fernandez-Garcia, M; (Eds.) 2007. Synthesis, Properties, Applications of Oxide Nanoparticle. Whiley: New Jersy.104, 4063..
[13] Senthilkumar, S.R. and Sivakumar, T. 2014. Green Tea (Camellia sinensis) mediated synthesis of Zinc oxide (Zno) nanopartilces and studies on their antimicrobial activities. International Journal of Pharamacy and Pharmaceutical Sciences, 6(6): 55-60.
[14] Sewani, S and Qureshi, M. 2016. Antimicrobial activity of Neem, Clove, Curry leaves, Cardamom, Tulsi stem and Tulsi leaves. International Research Journal of Biological Sciences, 5(1): 42-46.
[15] Shanmugam, B., Shanmugam, K.R., Ravi, S., Subbaiah, V.G., Mallikarjuna, K. and Reddy, K.S. 2014. Antibacterial activity and Phytochemical screening of Phyllanthusniruriin ethanolic, methanolic and aqueous extracts. Int. J. Pharm. Sci. Rev. Res., 27 (2): 85-89.
[16] Sharma, N.C., Sahi, S.V., Nath, S., Parsons, J.G., Torresdey, G.J.L. and Pal, T. 2009. Synthesis of plant-mediated gold nanoparticle and catalytic role of Biomatrix-Embedded nanomaterials. Environment Science Technology, 41: 5137-5142.
[17] Sundararaj. 1997. Practical manual of Microbiology. 3(3):27-50
[18] Suresh Y, Annapurna S, Bhikshamaiah G and Singh A.K, 2014. Copper Nanoparticle Green synthesis and Characterization. International Journal of Scientific & Engineering Research. 5( 3 ): 156-160.
[19] TayyabaNaseem, Muhammad, and Akhyarfarrukh, 2015. Antibacterial activity of green synthesis of iron nanoparticle using Lawsoniainermis and Gardianajasminoides leaves extract Hindawi publishing corporation, Journal of chemistry, volume, Article ID 912342, 7 pages.
[20] Thakkar, K.N., Mhatre, S.S., Parikh, R.Y. 2010. Biological synthesis of metallic nanoparticle. Nanomedicine, 6 (2): 257-262.
[21] Willard, M.A., Kurihara, L.K., Carpenter, E.E., Calvin, S. and Harris, V.G. 2014. Chemically prepared magnetic nanoparticle. International Materials Reviews, 125-170.
[22] Wykoff, R. W. G. crystal structures, 2nd edition; wiley: new york, 1964.